normal human lung fibroblast cells (nhlf)  (Lonza)

Journal: Cancers

Article Title: Conventional Cancer Therapies Can Accelerate Malignant Potential of Cancer Cells by Activating Cancer-Associated Fibroblasts in Esophageal Cancer Models

doi: 10.3390/cancers15112971

Figure Lengend Snippet: Efficacy of chemotherapy or radiotherapy against cancer cells and fibroblasts. ( A , B ) Viability of human-derived fibroblasts ( A ) or cancer cells ( B ) induced by chemotherapy (CDDP or 5-FU) was measured using XTT metabolic activity. The control group (100 %) did not receive chemotherapy ( n = 4. Mean ± SD). ( C ) Viability of TE4 and FEF3 cells induced by radiotherapy measured using XTT ( n = 4. Mean ± SD). ( D ) Relative proliferation curves of FEF3 cells treated by radiotherapy. Day 0 is set as the control ( n = 4; mean ± SD). In ( A – C ), the representative examples from five experiments were shown, and the representative example from three experiments was shown in ( D ).

Article Snippet: NHLF, a normal human lung fibroblast cell line, was purchased from Cambrex Corporation (East Rutherford, NJ, USA), and WI-38, a human fetal lung fibroblast cell line, was purchased from the Health Science Research Resource Bank (Osaka, Japan).

Techniques: Derivative Assay, Activity Assay

Journal: Cancers

Article Title: Conventional Cancer Therapies Can Accelerate Malignant Potential of Cancer Cells by Activating Cancer-Associated Fibroblasts in Esophageal Cancer Models

doi: 10.3390/cancers15112971

Figure Lengend Snippet: Phenotypic changes in normal fibroblasts after chemotherapy. ( A ) Treatment schedule. ( B ) Fluorescent microscopic images of human fibroblasts (FEF3, NHLF, and WI-38) after chemotherapy (CDDP or 5-FU). FAP (green), α-SMA (red), and DAPI (blue) staining is shown. Scale bar, 20 µm. The representative examples from four experiments were shown. ( C , D ) Flow cytometric analysis of FAP and α-SMA expression in the three fibroblast cell lines. Representative flow cytometric histograms are shown ( C ), and the relative fluorescence intensity (RFI) was calculated (( D ), n = 4. Mean ± SD).

Article Snippet: NHLF, a normal human lung fibroblast cell line, was purchased from Cambrex Corporation (East Rutherford, NJ, USA), and WI-38, a human fetal lung fibroblast cell line, was purchased from the Health Science Research Resource Bank (Osaka, Japan).

Techniques: Staining, Expressing, Fluorescence

Journal: Cancers

Article Title: Conventional Cancer Therapies Can Accelerate Malignant Potential of Cancer Cells by Activating Cancer-Associated Fibroblasts in Esophageal Cancer Models

doi: 10.3390/cancers15112971

Figure Lengend Snippet: Phenotypic changes in fibroblasts following radiotherapy. ( A ) Treatment schedule. ( B ) Fluorescent microscopic images of human-derived fibroblasts (FEF3, NHLF, and WI-38) after radiotherapy (16 Gy/8 fx). FAP (green), α-SMA (red), and DAPI (blue) staining is shown. The Sham group is used as the control group. Scale bar, 20 µm. The representative examples from four experiments were shown. ( C , D ) Flow cytometric analysis of FAP and α-SMA expression in the three fibroblast cell lines. Representative flow cytometric histograms are shown ( C ), and the RFI was calculated (( D ), n = 4. Mean ± SD).

Article Snippet: NHLF, a normal human lung fibroblast cell line, was purchased from Cambrex Corporation (East Rutherford, NJ, USA), and WI-38, a human fetal lung fibroblast cell line, was purchased from the Health Science Research Resource Bank (Osaka, Japan).

Techniques: Derivative Assay, Staining, Expressing